Protein
Protein Name Thymidylate synthase
#PDB ID 13
Organism Escherichia coli (strain K12)
Uniprot ID/ACC P0A884 (TYSY_ECOLI)
Synonym(s)
  •   AJR21_RS13215
  • AJR21_013430
show 1 synonym(s)
Gene Symbol thyA
Gene ID 58461970
Sequence MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPLVTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWPTPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLSCQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHLQLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI
Protein Class TRANSFERASE
Function Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product (PubMed:3286637, PubMed:2223754, PubMed:9826509). This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis. This protein also binds to its mRNA thus repressing its own translation (PubMed:7708505).
Bound Ligands
Ligand ID HET Code PDB ID Binding Affinity Binding Database Structure Complex Card
COVPDB1415 UFP 1TSN Ki : 10 nM BindingDB SHOW
COVPDB1415 UFP 1BJG
-
-
SHOW
COVPDB1422 UMC 6CDZ
-
-
SHOW
COVPDB1422 UMC 6NNR
-
-
SHOW
COVPDB1141 NDU 3BFI
-
-
SHOW
COVPDB1422 UMC 4GEV
-
-
SHOW
COVPDB1141 NDU 3BHR
-
-
SHOW
COVPDB1393 TP2 1F4C
-
-
SHOW
COVPDB1141 NDU 3BHL
-
-
SHOW
COVPDB1393 TP2 1F4D
-
-
SHOW
COVPDB1422 UMP 1KZI
-
-
SHOW
COVPDB1141 NDU 3B9H
-
-
SHOW
COVPDB1422 UMP 1TRG
-
-
SHOW